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baf.a (Fisher Scientific)

Name: baf.a
Brand: Fisher Scientific
Rating: 90 (1 reviews)

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Fisher Scientific baf.a
Cells were exposed with 3-MA (10 mM) <t>and</t> <t>Baf.A</t> (1 nM) alone or with gefitinb (5 μM) for 48 hours, and the inhibitory were confirmed by immunofluorescence staining. LC3 puncta in the cells were detected by immunofluorescence under confocal laser microscopy in MDA-MB-231 (A) and MDA-MB-468 (B).

Cells were exposed with 3-MA (10 mM) <t>and</t> <t>Baf.A</t> (1 nM) alone or with gefitinb (5 μM) for 48 hours, and the inhibitory were confirmed by immunofluorescence staining. LC3 puncta in the cells were detected by immunofluorescence under confocal laser microscopy in MDA-MB-231 (A) and MDA-MB-468 (B).

Baf.A, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/baf.a/product/Fisher Scientific
Average 90 stars, based on 1 article reviews

baf.a - by Bioz Stars, 2026-03

90/100 stars

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1) Product Images from "Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer"

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

Journal: PLoS ONE

doi: 10.1371/journal.pone.0177694

Cells were exposed with 3-MA (10 mM) and Baf.A (1 nM) alone or with gefitinb (5 μM) for 48 hours, and the inhibitory were confirmed by immunofluorescence staining. LC3 puncta in the cells were detected by immunofluorescence under confocal laser microscopy in MDA-MB-231 (A) and MDA-MB-468 (B).

Figure Legend Snippet: Cells were exposed with 3-MA (10 mM) and Baf.A (1 nM) alone or with gefitinb (5 μM) for 48 hours, and the inhibitory were confirmed by immunofluorescence staining. LC3 puncta in the cells were detected by immunofluorescence under confocal laser microscopy in MDA-MB-231 (A) and MDA-MB-468 (B).

Techniques Used: Immunofluorescence, Staining, Microscopy

$(A) MDA-MB-231 and MDA-MB-468 cells were treated with 0, 1.25, 2.50, 5.00, 10.00, 20.00 μM Ge alone or combined with 3-MA (10 mM) or Baf.A (1 nM) respectively for 48 hours, DMSO acted as the control, and then subjected to CCK8 assay. Absorbance value was calculated and standardized to DMSO group. Three independent experiments were performed. (B) The above cells were treated with DMSO (0.2%), 3-MA (10 mM), Baf.A (1 nM), Ge (5 μM), Ge (5 μM) +3-MA (10 mM) and Ge (5 μM) +Baf.A (1 nM), DMSO acted as the control, and subjected to cell colony formation assay. (C) Cell surviving fraction were calculated and presented as mean ± SD; *p < 0.05. Three independent experiments were performed.$
Figure Legend Snippet: (A) MDA-MB-231 and MDA-MB-468 cells were treated with 0, 1.25, 2.50, 5.00, 10.00, 20.00 μM Ge alone or combined with 3-MA (10 mM) or Baf.A (1 nM) respectively for 48 hours, DMSO acted as the control, and then subjected to CCK8 assay. Absorbance value was calculated and standardized to DMSO group. Three independent experiments were performed. (B) The above cells were treated with DMSO (0.2%), 3-MA (10 mM), Baf.A (1 nM), Ge (5 μM), Ge (5 μM) +3-MA (10 mM) and Ge (5 μM) +Baf.A (1 nM), DMSO acted as the control, and subjected to cell colony formation assay. (C) Cell surviving fraction were calculated and presented as mean ± SD; *p < 0.05. Three independent experiments were performed.

Techniques Used: CCK-8 Assay, Colony Assay

MDA-MB-468 xenograft tumor was established and treated as follows: DMSO, 3-MA (1.0 mg/kg), Baf.A (1.0 mg/kg), Ge (100 mg/kg), Ge (100 mg/kg) +3-MA (1.0 mg/kg) and Ge (100 mg/kg) +Baf.A (1.0 mg/kg). 3-MA and Baf.A were injected intratumorally, Ge was administered via oral gavage. Tumor growth curves (A) , tumor weight (B) , tumor image (C) , and nude mouse image (D) with different treatment were detected. The results are shown as means ± SD; *p < 0.05.

Figure Legend Snippet: MDA-MB-468 xenograft tumor was established and treated as follows: DMSO, 3-MA (1.0 mg/kg), Baf.A (1.0 mg/kg), Ge (100 mg/kg), Ge (100 mg/kg) +3-MA (1.0 mg/kg) and Ge (100 mg/kg) +Baf.A (1.0 mg/kg). 3-MA and Baf.A were injected intratumorally, Ge was administered via oral gavage. Tumor growth curves (A) , tumor weight (B) , tumor image (C) , and nude mouse image (D) with different treatment were detected. The results are shown as means ± SD; *p < 0.05.

Techniques Used: Injection

Cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours. The cycle distributions of MDA-MB-231 and MDA-MB-468 cells were analyzed.

Figure Legend Snippet: Cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours. The cycle distributions of MDA-MB-231 and MDA-MB-468 cells were analyzed.

Techniques Used:

Cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours. Phospho-ATM, Phospho-Chk1, Phospho-Chk2, γ-H2AX, and ACTB of MDA-MB-231 and MDA-MB-468 cells were analyzed by western blot.

Figure Legend Snippet: Cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours. Phospho-ATM, Phospho-Chk1, Phospho-Chk2, γ-H2AX, and ACTB of MDA-MB-231 and MDA-MB-468 cells were analyzed by western blot.

Techniques Used: Western Blot

The above cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours and were subjected to western blot using following antibodies, Cytochrome C (Cyto C), cleaved CASP3, BAX, Bcl-2 and ACTB (A) . Hematoxylin-eosin staining (B) and immunohistochemical assays (anti-cleaved CASP 3) (C) were performed in MDA-MB-468 xenograft.

Figure Legend Snippet: The above cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours and were subjected to western blot using following antibodies, Cytochrome C (Cyto C), cleaved CASP3, BAX, Bcl-2 and ACTB (A) . Hematoxylin-eosin staining (B) and immunohistochemical assays (anti-cleaved CASP 3) (C) were performed in MDA-MB-468 xenograft.

Techniques Used: Western Blot, Staining, Immunohistochemical staining

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Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: Cells were exposed with 3-MA (10 mM) and Baf.A (1 nM) alone or with gefitinb (5 μM) for 48 hours, and the inhibitory were confirmed by immunofluorescence staining. LC3 puncta in the cells were detected by immunofluorescence under confocal laser microscopy in MDA-MB-231 (A) and MDA-MB-468 (B).

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques: Immunofluorescence, Staining, Microscopy

Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: (A) MDA-MB-231 and MDA-MB-468 cells were treated with 0, 1.25, 2.50, 5.00, 10.00, 20.00 μM Ge alone or combined with 3-MA (10 mM) or Baf.A (1 nM) respectively for 48 hours, DMSO acted as the control, and then subjected to CCK8 assay. Absorbance value was calculated and standardized to DMSO group. Three independent experiments were performed. (B) The above cells were treated with DMSO (0.2%), 3-MA (10 mM), Baf.A (1 nM), Ge (5 μM), Ge (5 μM) +3-MA (10 mM) and Ge (5 μM) +Baf.A (1 nM), DMSO acted as the control, and subjected to cell colony formation assay. (C) Cell surviving fraction were calculated and presented as mean ± SD; *p < 0.05. Three independent experiments were performed.

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques: CCK-8 Assay, Colony Assay

Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: MDA-MB-468 xenograft tumor was established and treated as follows: DMSO, 3-MA (1.0 mg/kg), Baf.A (1.0 mg/kg), Ge (100 mg/kg), Ge (100 mg/kg) +3-MA (1.0 mg/kg) and Ge (100 mg/kg) +Baf.A (1.0 mg/kg). 3-MA and Baf.A were injected intratumorally, Ge was administered via oral gavage. Tumor growth curves (A) , tumor weight (B) , tumor image (C) , and nude mouse image (D) with different treatment were detected. The results are shown as means ± SD; *p < 0.05.

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques: Injection

Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: Cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours. The cycle distributions of MDA-MB-231 and MDA-MB-468 cells were analyzed.

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques:

Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: Cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours. Phospho-ATM, Phospho-Chk1, Phospho-Chk2, γ-H2AX, and ACTB of MDA-MB-231 and MDA-MB-468 cells were analyzed by western blot.

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: The above cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours and were subjected to western blot using following antibodies, Cytochrome C (Cyto C), cleaved CASP3, BAX, Bcl-2 and ACTB (A) . Hematoxylin-eosin staining (B) and immunohistochemical assays (anti-cleaved CASP 3) (C) were performed in MDA-MB-468 xenograft.

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques: Western Blot, Staining, Immunohistochemical staining

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1) Product Images from "Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer"

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